Partial Purification of Mucor pusillus Intracellular Proteases
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چکیده
منابع مشابه
Partial Purification of Mucor pusillus Intracellular Proteases.
The intracellular protease extracted from the freeze-dried mycelia obtained after the growth of Mucor pusillus at 30 degrees C in corn steep liquor medium was chromatographed on DEAE-A50. Some characteristics of the protease fractions obtained after ion-exchange chromatography were determined and compared with those of the extracellular proteases reported previously. The mycelia were found to c...
متن کاملPurification and properties of Mucor pusillus acid protease.
The protease produced by Mucor pusillus was recovered from a wheat bran medium by treatment with ammonium sulfate, ethyl alcohol, gel filtration and ion-exchange chromatography. The yield of the enzyme was 55%. The overall increase in the specific activity of the protease was 34-fold. The purified protease was most active at pH 3.8 and 5.6 against hemoglobin and casein, respectively. Optimal hy...
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Lipase of Mucor pusillus NRRL 2543 was recovered with ammonium sulfate precipitation, gel filtration on Sephadex G-75, and anion-exchange chromatography on diethylaminoethyl-Sephadex A-50. Maximal glycerol ester hydrolase (lipase) activity was observed at pH 5.0 to 5.5 and 50 C when trioctanoin and olive oil were used as substrates. The enzyme also showed esterase activity; it hydrolyzed, with ...
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Introduction 1.1.20) catalyze the hydrolysis of ester and depside bonds in tannic acid [1], the reaction end products is glucose and gallic acid [2,3]. The enzyme is utilized in a number of industrial applications, is extensively used in wine, beer, coffee flavored soft drinks [4] and also including the manufacture of instant tea [5]. In addition gallic acid, finds use in the dye making, and le...
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Y75N mutant Mucor pusillus pepsin has been overexpressed in yeast, purified and cocrystallized with the iodine-containing human renin inhibitor CP-113972 [(2R,3S]-isopropyl 3-[(L-prolyl-p-iodo-L-phenylalanyl-S-methyl-cysteinyl)amino-4]-cyclohexyl-2-hydroxybutanoate] for X-ray crystallography. Tetragonal complex crystals with space group P4(3)2(1)2 were produced by the hanging-drop vapour-diffus...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1983
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.45.1.94-96.1983